Not run tubeless on the road bike, but had one on for 2 years on the mtb, just took it off there are signs of sealing holes in a couple of places but I don't recall the punctures it must have been a near instant seal, the sealant was still liquid & had not dried out, but I had topped it up previously, this tryre was set up using a 20" inner tube stretched & slit down the centre then the excess trimmed off when the tyre fitted, (known as Ghetto fitting) I was having trouble infating the tyre with just rim tape, these are Bontrager tubeless ready rims, I have just built up some wheels with Stans Crest rims and the tyres pumped up 1st time with a track pump, just Stans rim tape and a tubless valve on these rims.