When do you feel the burn?

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Personally, seems to be around 40/45 miles. I mean I feel the 'burn' on most hills (sorry, inclines!) but once I get to 40/45 it seems to become a struggle. It still varies though...did a ride with a mate couple of week ago, trying to do a 50 but got to 45 and couldn't really mange anymore, got home at 48...but then 3 days later went out by myself and did 54, pain free, hassle free etc (which was my longest ride)...weird

I do however often find the first mile or two a struggle, just legs being tight/stiff I guess, and easy enough to pedal through them but always makes me think its gonna be a bad day...
 
Depends a lot on the intensity and the terrain/ conditions to me. I could go a 100 miles and feel no burn but after a 100m uphill into a headwind at speed I could feel the burn.
 
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